Effects of antibiotic concentration and nutrient medium ... | 動物用藥查詢網
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由LCGomes著作·2017·被引用15次—Although30μgmL−1kanamycinistheworkingconcentrationindicatedforplasmidsderivedfrompETvectors(Novagen2005;Carapuçaetal.
![Effects of antibiotic concentration and nutrient medium ...](https://i.imgur.com/TbfVXFL.jpg)
Recombinant protein production processes have to maximise yield while minimising cost, which involves balancing plasmid maintenance with cell growth and protein expression. The aim of this study was to analyse the influence of two factors on heterologous protein production in Escherichia coli biofilm cells—the concentration of antibiotic used to maintain the selective pressure and the nutrient medium composition. Escherichia coli JM109(DE3) cells transformed with plasmid pFM23 for enhanced green fluorescent protein (eGFP) expression and containing a kanamycin resistance gene were used. They were exposed to 20 or 30 μg mL−1 kanamycin during biofilm growth in two different culture media, a diluted medium (DM) or the lysogeny broth (LB). The higher antibiotic concentration increased the specific eGFP production in planktonic cells, wherea...
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